| Product name: |
HLA-DO rabbit pAb |
| Reactivity: |
Human |
| Alternative Names: |
HLA-DOB; HLA class II histocompatibility antigen; DO beta chain; MHC class II antigen DOB |
| Source: |
Rabbit |
| Dilutions: |
Western Blot: 1/500 – 1/2000. ELISA: 1/40000. Not yet tested in other applications. |
| Immunogen: |
The antiserum was produced against synthesized peptide derived from human HLA-DOB. AA range:1-50 |
| Storage: |
-20C/1 year |
| Clonality: |
Polyclonal |
| Isotype: |
IgG |
| Concentration: |
1 mg/ml |
| Observed Band: |
30kD |
| GeneID: |
3112 |
| Human Swiss-Prot No: |
P13765 |
| Cellular localization: |
Endosome membrane; Single-pass type I membrane protein. Lysosome membrane; Single-pass type I membrane protein. Complexes with HLA-DM molecule during intracellular transport and in endosomal/lysosomal compartments. Heterotetramerization is necessary to exit the ER. |
| Background: |
HLA-DOB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DOA) and a beta chain (DOB), both anchored in the membrane. It is located in intracellular vesicles. DO suppresses peptide loading of MHC class II molecules by inhibiting HLA-DM. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. [provided by RefSeq, Jul 2008], |
