| Product name: |
MCM4 (phospho Ser54) rabbit pAb |
| Reactivity: |
Human;Mouse;Monkey |
| Alternative Names: |
MCM4; CDC21; DNA replication licensing factor MCM4; CDC21 homolog; P1-CDC21 |
| Source: |
Rabbit |
| Dilutions: |
Western Blot: 1/500 – 1/2000. Immunohistochemistry: 1/100 – 1/300. ELISA: 1/20000. Not yet tested in other applications. |
| Immunogen: |
The antiserum was produced against synthesized peptide derived from human MCM4 around the phosphorylation site of Ser54. AA range:20-69 |
| Storage: |
-20C/1 year |
| Clonality: |
Polyclonal |
| Isotype: |
IgG |
| Concentration: |
1 mg/ml |
| Observed Band: |
85kD |
| GeneID: |
4173 |
| Human Swiss-Prot No: |
P33991 |
| Cellular localization: |
Nucleus . Chromosome . Associated with chromatin before the formation of nuclei and detaches from it as DNA replication progresses. . |
| Background: |
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. This gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks. Alternatively spliced transcri |
