| Product name: |
Rad17 (phospho Ser645) rabbit pAb |
| Reactivity: |
Human;Mouse |
| Alternative Names: |
RAD17; R24L; Cell cycle checkpoint protein RAD17; hRad17; RF-C/activator 1 homolog |
| Source: |
Rabbit |
| Dilutions: |
WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 |
| Immunogen: |
The antiserum was produced against synthesized peptide derived from human RAD17 around the phosphorylation site of Ser645. AA range:621-670 |
| Storage: |
-20C/1 year |
| Clonality: |
Polyclonal |
| Isotype: |
IgG |
| Concentration: |
1 mg/ml |
| Observed Band: |
77kD |
| GeneID: |
5884 |
| Human Swiss-Prot No: |
O75943 |
| Cellular localization: |
Nucleus . Phosphorylated form redistributes to discrete nuclear foci upon DNA damage. |
| Background: |
The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by the checkpoint kinase ATR following damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Multiple alternatively spliced transcript variants of this gene, which encode four distinct protein isoforms, h |
