| Product name: |
LIMK-1/2 (phospho Thr508/505) rabbit pAb |
| Reactivity: |
Human;Mouse;Rat |
| Alternative Names: |
LIMK1; LIMK; LIM domain kinase 1; LIMK-1; LIMK2; LIM domain kinase 2; LIMK-2 |
| Source: |
Rabbit |
| Dilutions: |
WB 1:500-2000;IHC-p 1:100-500;IF/ICC 1:100-500;ELISA 1:5000-20000 |
| Immunogen: |
Synthesized phospho-peptide around the phosphorylation site of human LIMK-1/2 (phospho Thr508/505) |
| Storage: |
-20C/1 year |
| Clonality: |
Polyclonal |
| Isotype: |
IgG |
| Concentration: |
1 mg/ml |
| Observed Band: |
72kD |
| GeneID: |
3984/3985 |
| Human Swiss-Prot No: |
P53667/P53671 |
| Cellular localization: |
Cytoplasm . Nucleus . Cytoplasm, cytoskeleton . Cell projection, lamellipodium . Predominantly found in the cytoplasm. Localizes in the lamellipodium in a CDC42BPA, CDC42BPB and FAM89B/LRAP25-dependent manner. . |
| Background: |
There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is a serine/threonine kinase that regulates actin polymerization via phosphorylation and inactivation of the actin binding factor cofilin. This protein is ubiquitously expressed during development and plays a role in many cellular processes associated with cytoskeletal structure. This protein also stimulates axon growth and may play a role in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cog |
