Human FETUB(Fetuin B) ELISA Kit – ATO2227
ATO2227
Size:Price:
48 T$320.00
96 T$458.00
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Overview

Product name: Human FETUB(Fetuin B) ELISA Kit
Reactivity: Human
Alternative Names: FETU-B; 16G2; Gugu; IRL685; Fetuin-like protein IRL685
Assay Type: Sandwich
Sensitivity: 58 pg/mL
Standard: 8000 pg/mL
Detection Range: 125-8000 pg/mL
Sample Type: Serum, plasma and other biological fluids
Assay Length: 3.5h
Research Area: Metabolic pathway;Infection immunity;Endocrinology;Bone metabolism;
Uniprot ID: Q9UGM5
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human FETUB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human FETUB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human FETUB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human FETUB in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
8000.00 2.268 2.195
4000.00 1.647 1.574
2000.00 1.187 1.114
1000.00 0.895 0.822
500.00 0.562 0.489
250.00 0.389 0.316
125.00 0.168 0.095
0.00 0.073 0.000

Precision

Intra-assay Precision (Precision within an assay):CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays):CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant FETUB and the recovery rates were calculated by comparing the measured value to the expected amount of FETUB in samples.
Matrix Recovery range Average
serum(n=5) 84-97% 90%
EDTA plasma(n=5) 82-94% 88%
Heparin plasma(n=5) 81-97% 89%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of FETUB and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 86-94% 93-102% 89-97% 85-94%
EDTA plasma(n=5) 89-99% 83-99% 98-105% 96-101%
Heparin plasma(n=5) 85-92% 79-96% 89-101% 85-97%