Mouse MMP1(Matrix Metalloproteinase 1) ELISA Kit – ATO2543
ATO2543
Size:Price:
48 T$320.00
96 T$458.00
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Overview

Product name: Mouse MMP1(Matrix Metalloproteinase 1) ELISA Kit
Reactivity: Mouse
Alternative Names: CLGN; CLG1; Collagenase; Interstitial Collagenase; Vertebrate Collagenase; Fibroblast Collagenase
Assay Type: Sandwich
Sensitivity: 27.4 pg/mL
Standard: 4000 pg/mL
Detection Range: 62.5-4000 pg/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 3.5h
Research Area: Tumor immunity;Infection immunity;Cardiovascular biology;Rheumatology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse MMP1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse MMP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse MMP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Mouse MMP1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
4000.00 1.996 1.914
2000.00 1.583 1.501
1000.00 1.144 1.062
500.00 0.933 0.851
250.00 0.594 0.512
125.00 0.312 0.230
62.50 0.165 0.083
0.00 0.082 0.000

Precision

Intra-assay Precision (Precision within an assay):CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays):CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant MMP1 and the recovery rates were calculated by comparing the measured value to the expected amount of MMP1 in samples.
Matrix Recovery range Average
serum(n=5) 80-95% 87%
EDTA plasma(n=5) 86-99% 92%
Heparin plasma(n=5) 82-97% 89%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MMP1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 88-97% 93-103% 94-106% 91-101%
EDTA plasma(n=5) 88-101% 93-106% 85-95% 92-104%
Heparin plasma(n=5) 82-96% 95-102% 87-98% 81-97%