Guinea pig HB(Hemoglobin) ELISA Kit – ATO1353
ATO1353
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48 T$370.00
96 T$528.00
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Overview

Product name: Guinea pig HB(Hemoglobin) ELISA Kit
Reactivity: Guinea pig
Alternative Names: Hgb; Haemoglobin; Heterotetramer()2
Assay Type: Sandwich
Sensitivity: 4.58 ng/mL
Standard: 800 ng/mL
Detection Range: 12.5-800 ng/mL
Sample Type: Serum, plasma, erythrocyte lysates.
Assay Length: 3.5h
Research Area: Metabolic pathway;Infection immunity;Hematology;Neuro science;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Guinea pig HB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Guinea pig HB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Guinea pig HB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Guinea pig HB in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
800.00 2.121 2.039
400.00 1.731 1.649
200.00 1.106 1.024
100.00 0.812 0.730
50.00 0.521 0.439
25.00 0.332 0.250
12.50 0.225 0.143
0.00 0.082 0.000

Precision

Intra-assay Precision (Precision within an assay):CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays):CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant HB and the recovery rates were calculated by comparing the measured value to the expected amount of HB in samples.
Matrix Recovery range Average
serum(n=5) 82-95% 88%
EDTA plasma(n=5) 80-97% 88%
Heparin plasma(n=5) 88-99% 93%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HB and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 90-97% 96-102% 91-103% 89-97%
EDTA plasma(n=5) 95-104% 87-96% 92-101% 82-90%
Heparin plasma(n=5) 78-92% 83-96% 87-98% 95-102%