Cattle CST3(Cystatin 3) ELISA Kit – ATO7743
ATO7743
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48 T$412.00
96 T$588.00
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OverviewPublications

Product name: Cattle CST3(Cystatin 3) ELISA Kit
Reactivity: Cattle
Alternative Names: Cys-C; Cystatin C; Gamma Trace; Post-Gamma-Globulin; Neuroendocrine Basic Polypeptide; Amyloid Angiopathy And Cerebral Hemorrhage
Assay Type: Sandwich
Sensitivity: 33 pg/mL
Standard: 5000 pg/mL
Detection Range: 78.13-5000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Kidney biomarker;Urology;
Uniprot ID: P01035
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle CST3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle CST3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle CST3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Cattle CST3 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
5000.00 2.056 1.971
2500.00 1.594 1.509
1250.00 1.142 1.057
625.00 0.806 0.721
312.50 0.453 0.368
156.25 0.308 0.223
78.13 0.135 0.050
0.00 0.085 0.000

Precision

Intra-assay Precision (Precision within an assay):CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays):CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant CST3 and the recovery rates were calculated by comparing the measured value to the expected amount of CST3 in samples.
Matrix Recovery range Average
serum(n=5) 78-90% 84%
EDTA plasma(n=5) 95-107% 101%
Heparin plasma(n=5) 90-105% 97%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CST3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 89-97% 85-101% 84-98% 85-93%
EDTA plasma(n=5) 93-104% 87-96% 82-95% 78-99%
Heparin plasma(n=5) 79-92% 89-102% 87-98% 80-92%