Rat CX3CR1(Chemokine C-X3-C-Motif Receptor 1) ELISA Kit – ATO8787
ATO8787
Size:Price:
48 T$320.00
96 T$458.00
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Overview

Product name: Rat CX3CR1(Chemokine C-X3-C-Motif Receptor 1) ELISA Kit
Reactivity: Rat
Alternative Names: CCRL1; CMKBRL1; CMKDR1; GPR13; GPRV28; V28; Beta chemokine receptor-like 1; Fractalkine receptor; G-protein coupled receptor 13
Assay Type: Sandwich
Sensitivity: 9.21 pg/mL
Standard: 1500 pg/mL
Detection Range: 23.44-1500 pg/mL
Sample Type: tissue homogenates, cell lysates and other biological fluids
Assay Length: 3.5h
Research Area: CD & Adhesion molecule;
Uniprot ID: P35411
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CX3CR1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CX3CR1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CX3CR1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Rat CX3CR1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
1500.00 2.045 1.949
750.00 1.674 1.578
375.00 1.213 1.117
187.50 0.752 0.656
93.75 0.591 0.495
46.88 0.313 0.217
23.44 0.165 0.069
0.00 0.096 0.000

Precision

Intra-assay Precision (Precision within an assay):CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays):CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant CX3CR1 and the recovery rates were calculated by comparing the measured value to the expected amount of CX3CR1 in samples.
Matrix Recovery range Average
serum(n=5) 82-95% 88%
EDTA plasma(n=5) 83-98% 91%
Heparin plasma(n=5) 91-99% 95%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CX3CR1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 86-95% 93-103% 87-98% 85-96%
EDTA plasma(n=5) 92-105% 85-101% 85-94% 87-96%
Heparin plasma(n=5) 85-92% 83-96% 93-102% 87-98%