IP antibody use:3ug Flag Mouse IgG1 per ml Lysate, WB 1:5000 1,untransfected 293 cell lysate 2,transfected 293 cell lysate with C-Myc-tag fusion protein 3,IP (transfected 293+ normal Mouse IgG+Protein G agarose) 4,IP (transfected 293+anti- C-Myc mAb+ P