fT4(Free Thyroxine) ELISA Kit – ATO3119
ATO3119
Size:Price:
48 T$265.00
96 T$376.00
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Overview

Product name: fT4(Free Thyroxine) ELISA Kit
Reactivity: General
Alternative Names: FTI
Assay Type: Competitive Inhibition
Sensitivity: 0.48 pg/mL
Standard: 90 pg/mL
Detection Range: 1.41-90 pg/mL
Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 2h
Research Area: Signal transduction;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with fT4 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to fT4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of fT4 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
90.00 0.212
45.00 0.386
22.50 0.596
11.25 0.812
5.63 1.112
2.82 1.537
1.41 1.697
0.00 2.213

Precision

Intra-assay Precision (Precision within an assay):CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays):CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant fT4 and the recovery rates were calculated by comparing the measured value to the expected amount of fT4 in samples.
Matrix Recovery range Average
serum(n=5) 88-107% 94%
EDTA plasma(n=5) 97-105% 101%
Heparin plasma(n=5) 87-99% 92%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of fT4 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 79-93% 89-97% 92-105% 83-92%
EDTA plasma(n=5) 85-100% 80-97% 81-92% 87-103%
Heparin plasma(n=5) 95-104% 83-96% 95-102% 87-98%