IP antibody use:5ug HA Mouse IgG1 per ml Lysate, WB 1:5000 1,untransfected 293 cell lysate 2,transfected 293 cell lysate with HA-tag fusion protein 3,IP(untransfted 293+anti-HA mAb+Protein G agarose) 4,IP (transfected 293+ normal Mouse IgG+Protein G ag