Human NT-ProANP(N-Terminal Pro-Atrial Natriuretic Peptide) ELISA Kit – ATO2399
ATO2399
Size:Price:
48 T$320.00
96 T$458.00
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Overview

Product name: Human NT-ProANP(N-Terminal Pro-Atrial Natriuretic Peptide) ELISA Kit
Reactivity: Human
Alternative Names: NT-Pro-ANP; N-ANP
Assay Type: Sandwich
Sensitivity: 14 pg/mL
Standard: 2500 pg/mL
Detection Range: 39.07-2500 pg/mL
Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Endocrinology;Cardiovascular biology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NT-ProANP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NT-ProANP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NT-ProANP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human NT-ProANP in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
2500.00 2.054 1.966
1250.00 1.624 1.536
625.00 1.073 0.985
312.50 0.839 0.751
156.25 0.518 0.430
78.13 0.385 0.297
39.07 0.216 0.128
0.00 0.088 0.000

Precision

Intra-assay Precision (Precision within an assay):CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays):CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant NT-ProANP and the recovery rates were calculated by comparing the measured value to the expected amount of NT-ProANP in samples.
Matrix Recovery range Average
serum(n=5) 80-94% 87%
EDTA plasma(n=5) 89-101% 95%
Heparin plasma(n=5) 82-95% 88%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NT-ProANP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 93-102% 78-92% 87-98% 80-93%
EDTA plasma(n=5) 88-97% 86-92% 95-103% 98-106%
Heparin plasma(n=5) 95-102% 87-101% 82-91% 85-97%