| Product name: |
MDC1 (phospho Ser513) rabbit pAb |
| Reactivity: |
Human;Rat;Mouse; |
| Alternative Names: |
MDC1; KIAA0170; NFBD1; Mediator of DNA damage checkpoint protein 1; Nuclear factor with BRCT domains 1 |
| Source: |
Rabbit |
| Dilutions: |
Immunohistochemistry: 1/100 – 1/300. ELISA: 1/5000. Not yet tested in other applications. |
| Immunogen: |
The antiserum was produced against synthesized peptide derived from human MDC1 around the phosphorylation site of Ser513. AA range:479-528 |
| Storage: |
-20C/1 year |
| Clonality: |
Polyclonal |
| Isotype: |
IgG |
| Concentration: |
1 mg/ml |
| Molecular Weight: |
227kD |
| GeneID: |
9656 |
| Human Swiss-Prot No: |
Q14676 |
| Cellular localization: |
Nucleus . Chromosome . Associated with chromatin. Relocalizes to discrete nuclear foci following DNA damage, this requires ‘Ser-139’ phosphorylation of H2AX. Colocalizes with APTX at sites of DNA double-strand breaks. |
| Background: |
The protein encoded by this gene contains an N-terminal forkhead domain, two BRCA1 C-terminal (BRCT) motifs and a central domain with 13 repetitions of an approximately 41-amino acid sequence. The encoded protein is required to activate the intra-S phase and G2/M phase cell cycle checkpoints in response to DNA damage. This nuclear protein interacts with phosphorylated histone H2AX near sites of DNA double-strand breaks through its BRCT motifs, and facilitates recruitment of the ATM kinase and meiotic recombination 11 protein complex to DNA damage foci. [provided by RefSeq, Jul 2008], |
