| Product name: |
p40-phox (phospho Thr154) rabbit pAb |
| Reactivity: |
Human;Mouse |
| Alternative Names: |
NCF4; SH3PXD4; Neutrophil cytosol factor 4; NCF-4; Neutrophil NADPH oxidase factor 4; SH3 and PX domain-containing protein 4; p40-phox; p40phox |
| Source: |
Rabbit |
| Dilutions: |
WB 1:500-2000 ,Immunohistochemistry: 1/100 – 1/300. ELISA: 1/5000. Not yet tested in other applications. |
| Immunogen: |
The antiserum was produced against synthesized peptide derived from human p40 phox around the phosphorylation site of Thr154. AA range:120-169 |
| Storage: |
-20C/1 year |
| Clonality: |
Polyclonal |
| Isotype: |
IgG |
| Concentration: |
1 mg/ml |
| Molecular Weight: |
39kD |
| GeneID: |
4689 |
| Human Swiss-Prot No: |
Q15080 |
| Cellular localization: |
Cytoplasm, cytosol . Endosome membrane ; Peripheral membrane protein ; Cytoplasmic side . Membrane ; Peripheral membrane protein . |
| Background: |
The protein encoded by this gene is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding d |
