Rat EMMPRIN(Extracellular Matrix Metalloproteinase Inducer) ELISA Kit – ATO7006
ATO7006
Size:Price:
48 T$320.00
96 T$458.00
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Overview

Product name: Rat EMMPRIN(Extracellular Matrix Metalloproteinase Inducer) ELISA Kit
Reactivity: Rat
Alternative Names: CD147; BSG; 5F7; M6; OK; TCSF; Basigin(Ok Blood Group); Collagenase stimulatory factor; OK blood group antigen; Tumor cell-derived collagenase stimulatory factor
Assay Type: Sandwich
Sensitivity: 5.5 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: Serum, plasma, lung lavage fluid and other biological fluids.
Assay Length: 3.5h
Research Area: CD & Adhesion molecule;Tumor immunity;Infection immunity;Reproductive science;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat EMMPRIN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat EMMPRIN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat EMMPRIN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Rat EMMPRIN in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
1000.00 2.126 2.040
500.00 1.784 1.698
250.00 1.232 1.146
125.00 0.813 0.727
62.50 0.597 0.511
31.25 0.325 0.239
15.63 0.207 0.121
0.00 0.086 0.000

Precision

Intra-assay Precision (Precision within an assay):CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays):CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant EMMPRIN and the recovery rates were calculated by comparing the measured value to the expected amount of EMMPRIN in samples.
Matrix Recovery range Average
serum(n=5) 95-107% 101%
EDTA plasma(n=5) 80-95% 87%
Heparin plasma(n=5) 80-93% 86%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of EMMPRIN and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 88-97% 86-97% 85-103% 89-97%
EDTA plasma(n=5) 92-102% 81-93% 86-98% 91-103%
Heparin plasma(n=5) 85-104% 83-96% 95-103% 88-95%