| Product name: |
Recombinant Mouse TGFBR2 (C-6His) |
| Description: |
Recombinant Mouse Transforming Growth Factor-beta Receptor Type II is produced by our Mammalian expression system and the target gene encoding Ile24-Asp159 is expressed with a 6His tag at the C-terminus. |
| Accession: |
Q62312-2 |
| Molecular weight: |
16.2 KDa |
| Apparent molecular weight: |
25-38 KDa, reducing conditions |
| Purity: |
Greater than 95% as determined by reducing SDS-PAGE. |
| Endotoxin: |
Less than 0.1 ng/g (1 EU/g) as determined by LAL test. |
| Biological activity: |
Measured by its ability to inhibit TGF-beta 1 activity on TF1 human erythroleukemic cells.The ED50 for this effect is 1680.39ng/ml in the presence of 1ng/ml of recombinant human TGF-beta 1. |
| Redissolve: |
Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100g/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Storage: |
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
| Delivery condition: |
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below. |
| Background: |
Transforming growth factor- (TGF-) is an essential regulator in the processes of development, cell proliferation, and extracellular matrix deposition. TGF- regulates cellular processes by binding to three high-affinity cell surface receptors: TGF- receptor type I (TGF–RI), TGF- receptor type II (TGF–RII), and TGF- receptor type III (TGF–RIII). TGF- RII is consists of a C-terminal protein kinase domain and an N-terminal ectodomain and belongs to transforming growth factor-beta (TGF-) receptor subfamily. TGF- RII has a protein kinase domain which can form a heterodimeric complex with another receptor protein and bind TGF-beta. This receptor/ligand complex phosphorylates protein will enter the nucleus and regulate the transcription of a subset of genes related to cell proliferation. |
