IP antibody use:5ug V5 Mouse IgG1 per ml Lysate, WB 1:5000 1,untransfected 293 cell lysate 2,transfected 293 cell lysate with V5-tag fusion protein 3,IP(untransfted 293+anti-V5 mAb+Protein G agarose) 4,IP (transfected 293+ normal Mouse IgG+Protein G ag